Randomized Complete Block Design (RCBD) is generally used to improve the ability to detect true differences between the treatments we are trying by eliminating the influence of other known variations (groups) of experimental errors. If the idea is applied to eliminate two sources of diversity by grouping in two directions, then the design is called the Latin Square Design (LSD). Thus, LSD is an experimental design with two grouping directions , namely row and column. The number of treatments is equal to the number of replications so that each row and column will contain all treatments. In this design, randomization is limited by grouping it into rows and columns, so that each row and column will only get one treatment.